4 research outputs found
Apoptotic and Immunosuppressive Effects of Turmeric Paste on 7, 12 Di Methyl Benz (a) Anthracene Induced Skin Tumor Model of Wistar Rat
Dietary components with potent anticancerous property are gaining attention as therapeutic agents due to low cost of therapy and minimal toxic effects. Turmeric is one such miracle spices of Indian and South Asian recipes with multiple medicinal properties. The anticarcinogenic properties of its active compound curcumin have been studied in detail. However, studies on the medicinal properties of crude turmeric used as dietary agents are lacking. Therefore, in this study we investigated the effects of dietary and topical crude turmeric paste on DMBA induced skin tumor of male Wistar rats. We observed the apoptotic effect of crude turmeric paste on DMBA induced tumor with depletion of T cells response. Our results demonstrated the significant expression of major pro-apoptotic genes like caspase-2, 3, 8, 9, PARP, and p53 and down regulation of major pro-inflammatory (NF-B) and pro-angiogenic factors and (VEGF) in turmeric treated tumor tissues. We also observed significant decrease in CD4(+), CD8(+), and Natural Killer cell population as compared to the untreated group
Differential proteomics approach to identify putative protective antigens of <i>Mycobacterium tuberculosis </i> presented during early stages of macrophage infection and their evaluation as DNA vaccines
429-439<span style="font-size:11.0pt;font-family:
" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Unsatisfactory performance of the existing BCG vaccines, especially against
the adult pulmonary disease, has urged the need for an effective vaccine
against tuberculosis (TB). In this study, we employed differential proteomics
to obtain a list of antigens as potential vaccine candidates. Bacterial
epitopes being presented at early stages on MHC class I and
class II molecules of macrophages infected with <i style="mso-bidi-font-style:
normal">Mycobacterium tuberculosis (M.
tb) were identified using iTRAQ labelling and reverse phase LC-MS/MS. The
putative vaccine candidates, thus identified, were tested as plasmid DNA
vaccines in mice to ascertain their protective efficacy against the aerosolized
M. tb challenge, based on their ability
to reduce the bacterial load in the lungs of infected mice. Here, we observed
that 4 out of the 17 selected antigens imparted significant protection against
the challenge of M. tb. The four
shortlisted antigens were further assessed in a more stringent guinea pig
model, where too, they demonstrated significant protection. It concludes that
combining a proteomics approach with the in vivo assessment of vaccine
candidates in animal models can be valuable in identifying new potential candidates
to expand the antigenic repertoire for novel vaccines against TB.</span